Anti-Viral Surfaces in the Fight against the Spread of Coronaviruses.

This review is conducted against the background of nanotechnology, which provides us with a chance to effectively combat the spread of coronaviruses, and which primarily concerns polyelectrolytes and their usability for obtaining protective function against viruses and as carriers for anti-viral agents, vaccine adjuvants, and, in particular, direct anti-viral activity. This review covers nanomembranes in the form of nano-coatings or nanoparticles built of natural or synthetic polyelectrolytes--either alone or else as nanocomposites for creating an interface with viruses. There are not a wide variety of polyelectrolytes with direct activity against SARS-CoV-2, but materials that are effective in virucidal evaluations against HIV, SARS-CoV, and MERS-CoV are taken into account as potentially active against SARS-CoV-2. Developing new approaches to materials as interfaces with viruses will continue to be relevant in the future.

Polyelectrolyte Membrane Nanocoatings Aimed at Personal Protective and Medical Equipment Surfaces to Reduce Coronavirus Spreading.

The study of the surface of membrane coatings constructed with adsorbed coronavirus (COV) was described to test their suitability for the antiviral activity for application in personal protective and medical equipment. The nanocoating based on polyethyleneimine (PEI) or polystyrene sulfonate (PSS) with metallic nanoparticles incorporated was investigated using the AFM technique. Moreover, the functioning of human lung cells in a configuration with the prepared material with the adsorbed coronavirus was studied using microscopic techniques and flow cytometry. The mean values of the percentage share of viable cells compared with the control differed by a maximum of 22%. The results showed that PEI and PSS membrane layer coatings, modified with chosen metallic nanoparticles (AuNPs, AgNPs, CuNPs, FeNPs) that absorb COV, could support lung cells' function, despite the different distribution patterns of COV on designed surfaces as well as immobilized lung cells. Therefore, the developed membrane nanocoatings can be recommended as material for biomedical applications, e.g., medical equipment surfaces to reduce coronavirus spreading, as they adsorb COV and simultaneously maintain the functioning of the eukaryotic cells.